Project 3140: P. T. Rühr, M. Lambertz. 2019. Surface contrast enhancement of integumentary structures in X-ray tomography. Journal of Anatomy. 235 (2):379-385.
Abstract
Micro‐computed tomography (μCT) has become standard in the biological sciences to reconstruct, display and analyse 3D models of all kinds of organisms. However, it is often impossible to capture fine details of the surface and the internal anatomy at the same time with sufficient contrast. Here we introduce a new approach for the selective contrast‐enhancement of integumentary surface structures. The method relies on conventional and readily available sputter coaters to cover the entire sample with a thin layer of gold atoms. This approach proved successful on a diverse array of plants and animals. On average, we achieved a 14.48‐fold gain of surface contrast (ranging from 2.42‐fold to 86.93‐fold) compared with untreated specimens. Even X‐ray‐transparent samples such as spider silk became accessible via μCT. This selective contrast‐enhancement, makes it possible to digitally reconstruct fine surface structures with low absorbance while the tissue‐dependent grey value resolution of the inner anatomy is maintained and remains fully visualisable. The methodology is suited for a broad scientific application across biology and other sciences employing (μ)CT, as well as educative and public outreach purposes.Read the article »
Article DOI: 10.1111/joa.13008
Project DOI: 10.7934/P3140, http://dx.doi.org/10.7934/P3140
This project contains |
---|
Download Project SDD File |
Currently Viewing:
MorphoBank Project 3140
MorphoBank Project 3140
- Creation Date:
22 March 2018 - Publication Date:
20 July 2019 - Project views: 41848
- Media downloads: 7
Authors' Institutions
- University of Cologne
- Rheinische Friedrich-Wilhelms-Universität Bonn (University of Bonn)
- Zoologisches Forschungsmuseum Alexander Koenig
Members
member name | taxa | specimens | media |
Peter T. Rühr Project Administrator | 9 | 9 | 61 |
Project has no matrices defined.
Project views
type | number of views | Individual items viewed (where applicable) |
Total project views | 41848 | |
Project overview | 1751 | |
Media views | 25957 | Media search (2793 views); M488303 (429 views); M595131 (373 views); M595132 (395 views); M595138 (383 views); M595141 (439 views); M595142 (423 views); M595145 (405 views); M595146 (373 views); M595147 (414 views); M595148 (420 views); M595163 (399 views); M662067 (414 views); M662068 (396 views); M662069 (379 views); M662070 (406 views); M662071 (401 views); M662072 (382 views); M662073 (405 views); M662074 (393 views); M662075 (389 views); M662076 (398 views); M662077 (391 views); M662078 (389 views); M662079 (365 views); M662080 (392 views); M662081 (363 views); M662082 (421 views); M662083 (393 views); M662084 (396 views); M662085 (405 views); M662086 (397 views); M662087 (406 views); M662088 (402 views); M662089 (385 views); M670124 (383 views); M673279 (380 views); M673280 (377 views); M673281 (402 views); M673282 (396 views); M673283 (418 views); M673284 (393 views); M673296 (388 views); M673297 (433 views); M673298 (376 views); M673299 (358 views); M673300 (362 views); M673301 (360 views); M673302 (344 views); M673303 (355 views); M673304 (325 views); M673305 (319 views); M673306 (404 views); M673307 (314 views); M673308 (325 views); M673309 (313 views); M673310 (322 views); M673311 (340 views); M673312 (309 views); M673314 (315 views); M673315 (322 views); M673313 (310 views); |
Bibliography | 653 | |
Specimen list | 7213 | |
Taxon list | 4336 | |
Views for media list | 1930 | |
Documents list | 8 |
Project downloads
type | number of downloads | Individual items downloaded (where applicable) |
Total downloads from project | 191 | |
Project downloads | 184 | |
Media downloads | 7 | M595131 (1 download); M662076 (1 download); M673280 (1 download); M673303 (1 download); M673298 (1 download); M673311 (1 download); M595132 (1 download); |