Project 3166: B. K. Penney, K. R. Ehresmann, K. J. Jordan, G. Rufo. 2020. Micro‐computed tomography of spicule networks in three genera of dorid sea‐slugs (Gastropoda: Nudipleura: Doridina) shows patterns of phylogenetic significance. Acta Zoologica. 101 (1):5-23.
Specimen: Onchidoris bilamellata (Linnaeus, 1767) (HMCZ:359013)
View: Cross section at rhinophores


Dorid nudibranchs (Gastropoda: Nudipleura) are a key taxon for studying the evolution and interaction of chemical defense, color patterns and feeding specialization, but we lack a robust phylogeny for hypothesis testing. To provide new morphological characters, we investigated the extensive interior spicule networks of several dorid taxa. We compared traditional staining methods vs micro computed tomography (?CT) of 31 specimens representing 10 species of Aldisa, Cadlina and Onchidoris. We found that ?CT offered a non-destructive view of sufficient resolution to study the gross morphology of networks, although images of fine structures were too grainy to allow robust comparisons. Network form did not vary within species and was consistent within genera. The three genera varied in several obvious characters, such as presence of a pleural sinus channel, large dorsal spicules, and multispicular tracts, as well as in relative size, shape and orientation of spicules. These characters, combined with those from the literature, supported recent molecular phylogenies that group Cadlina with Aldisa, and that question the monophyly of the Cryptobranchia. This suggests that these network forms will prove a fruitful source of phylogenetic characters with at least genus-level resolution.

Read the article »

Article DOI: 10.1111/azo.12266

Project DOI: 10.7934/P3166,
This project contains
  • 18 Media
  • 10 Taxa
  • 13 Specimens
Total size of project's media files: 39.56M

Download Project SDD File
Currently Viewing:
MorphoBank Project 3166

    Authors' Institutions

    • Saint Anselm College


    member name taxa specimens media media
    Brian Penney
    Project Administrator

    Project has no matrices defined.

    Project views

    type number of views Individual items viewed (where applicable)
    Total project views13045
    Project overview945
    Media views5569Media search (1483 views); M508283 (215 views); M508284 (267 views); M508307 (256 views); M508308 (262 views); M508309 (223 views); M508310 (210 views); M508311 (220 views); M508312 (210 views); M508313 (239 views); M508314 (253 views); M508315 (245 views); M508316 (208 views); M508317 (214 views); M508318 (218 views); M508319 (213 views); M508320 (208 views); M508321 (213 views); M508322 (212 views);
    Taxon list1470
    Specimen list3485
    Views for media list1220
    Documents list1

    Project downloads

    type number of downloads Individual items downloaded (where applicable)
    Total downloads from project138
    Project downloads133
    Media downloads5M508319 (1 download); M508311 (1 download); M508315 (1 download); M508307 (2 downloads);