Project 3386: S. Stöhr, E. G. Clark, B. Thuy, S.A.F. Darroch. 2019. Comparison of 2D SEM imaging with 3D micro-tomographic imaging for phylogenetic inference in brittle stars (Echinodermata: Ophiuroidea). Zoosymposia. 15:146-158.
Specimen: Ophiolepis superba (SMNH/EV:174869)
View: SEM of lateral arm plate, internal

Abstract

Recent efforts to reconstruct the phylogeny of brittle stars (ophiuroids) have shown the need for more objective and reproducible data collection methods than the traditional visual examination and verbal description of morphological characters. Complex skeletal structures may be better understood in three dimensions than in two dimensions obtained from techniques like scanning electron microscopy (SEM). We test this hypothesis using three types of three-dimensional tomographic imaging methods—lab-based micro-CT, X-ray microscopy and synchrotron-based tomography—to examine the morphology of ophiuroid arms, and compare them with two- dimensional data obtained from SEM. We describe the advantages and disadvantages of each instrument and set of parameters in terms of the ease and efficiency of data collection for morphometric analyses. We present new morphological observations obtained by digital sectioning of three-dimensional images that could not be achieved with SEM. Overall, our findings suggest that three-dimensional imaging has a high potential to address the gaps in knowledge of the internal ophiuroid skeleton, which will be pivotal to providing morphological characters that will aid in phylogenetic reconstructions.


Read the article »

Article DOI: 10.11646/zoosymposia.15.1.17

Project DOI: 10.7934/P3386, http://dx.doi.org/10.7934/P3386
This project contains
  • 17 Media
  • 6 Taxa
  • 13 Specimens
Total size of project's media files: 2048 MB

Download Project SDD File
Currently Viewing:
MorphoBank Project 3386


    Members

    member name taxa specimens media media
    notes
    Sabine Stöhr
    Project Administrator
    Last logged in 10/21/19
    613177


    Project has no matrices defined.



    Project views

    type number of views Individual items viewed (where applicable)
    Total project views350
    Project overview70
    Media views137Media search (45 views); M657129 (9 views); M657222 (6 views); M657201 (4 views); M657202 (7 views); M657203 (5 views); M657204 (5 views); M657205 (5 views); M657206 (5 views); M657207 (5 views); M657208 (5 views); M657209 (5 views); M657210 (5 views); M657213 (5 views); M657214 (5 views); M657219 (5 views); M657220 (6 views); M657223 (5 views);
    Views for media list27
    Specimen list79
    Taxon list29
    Bibliography8




    Project downloads

    type number of downloads Individual items downloaded (where applicable)
    Total downloads from project7
    Media downloads3M657129 (3 downloads);
    Project downloads4