Project 379: S. L. Boyer, A. A. Howe, N. W. Juergens, M. C. Hove. 2011. A DNA barcoding approach to identification of freshwater mussel larvae encysted on wild fishes. Journal of the North American Benthological Society. 30 (1):182-194.
Specimen: Leptodea fragilis (Macalester College/Drum1)

Abstract

We developed a multilocus deoxyribonucleic acid (DNA)-barcoding approach to identify newly transformed juvenile mussels collected from naturally infested fishes in a federally protected waterway that is home to a diverse mussel community, the St Croix River (Minnesota/Wisconsin, USA). We used new and publicly available data downloaded from GenBank to build reference databases for identified adult mussels. We assessed the efficacy of the mitochondrial loci cytochrome oxidase c subunit I (COI) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) for DNA barcoding. We concluded that the barcoding gap between average intra- and interspecific genetic distances is wider for ND1 than for COI, but both loci perform well for species identification in character-based phylogenetic analyses. Almost every species formed a monospecific clade with high bootstrap and posterior-probability support. We obtained newly transformed juvenile mussels by collecting individuals of 3 different fish species that were infested with unionid larvae. We held the fish in aquaria until the mussels emerged naturally. We then extracted DNA and sequenced our loci of interest. When sequences from the juveniles were included in phylogenetic analyses, they grouped with single species (or, in one case, a pair of closely related species) with high bootstrap and posterior-probability support. Identifying juveniles using morphology alone is difficult and, in some cases, impossible. Therefore, our approach will be useful to researchers interested in the relationship between unionid mussels and their fish hosts.


Read the article »

Project DOI: 10.7934/P379, http://dx.doi.org/10.7934/P379
This project contains
  • 10 Media
  • 7 Taxa
  • 9 Specimens
Total size of project's media files: 2.21M

Download Project SDD File
Currently Viewing:
MorphoBank Project 379
  • Creation Date:
    02 July 2010
  • Publication Date:
    11 January 2011
  • Project views: 28734

    Authors' Institutions

    • University of Minnesota

    • Macalester College



    Members

    member name taxa specimens media
    Sarah Boyer
    Project Administrator
    7910


    Project has no matrices defined.



    Project views

    type number of views Individual items viewed (where applicable)
    Total project views28734
    Project overview2263
    Media views10509Media search (3813 views); M49793 (700 views); M49770 (643 views); M49789 (619 views); M49785 (636 views); M49781 (760 views); M49775 (704 views); M49794 (587 views); M49803 (691 views); M49787 (820 views); M49791 (536 views);
    Taxon list7357
    Specimen list7668
    Bibliography937




    Project downloads

    type number of downloads Individual items downloaded (where applicable)
    Total downloads from project294
    Project downloads294